甲基丙烯酸环氧丙酯对金仓鼠胚胎细胞的转化作用

Transformation of golden syrian hamster embryo cells induced by glycidyl methacrylate(GMA)

应用金仓鼠胚胎（ＳＨＥ）细胞的转化试验的结果表明：当甲基丙烯酸环氧丙酯（ＧＭＡ）浓度在０．９～１４．２ｍｇ／Ｌ时，可诱发细胞的形态学转化，转化率范围是（０．８４～２．１８）×１０－５，最高的转化率与阳性对照３－甲基胆蒽（ＭＣＡ）转化率相近并呈剂量－效应关系；转化细胞对刀豆素凝集反应的能力增强；有在软琼脂中形成集落的能力。形态学转化的细胞已获得恶性转化的特征。

t has been demonstrated that glycidyl Methacrylate(GMA), a direct mutagen, can induce genotoxic damage in both somatic cells and germ cells. In order to approach, the carcinogenicity of GMA, the focus assay was used to indentify the transformation of freshly isolated diploid Syrian Hamster Embryo (SHE) cells. The frequency of focus formation ranged from 0.84×10 -5 to 2.18 foci/10 5 surviving cells with GMA concentrations from 0.9 to 14.2mg/L, respectively. Cells in a transformed focus exhibited three dimensioned growth and crossing over the periphery of the focus. These cells usually had an increased ratio of nucleus to cytoplasm and were more basophilic. Further evidences for the cell transformation, including the agglutinability following exposure to concanavatin A(Con A) and colony formation on the soft agar medium were also obtained in our experiments. These results revealed potential carcinogenesis of GMA in the animal.