析因设计在Cu和VE对CCl4所致肝损伤的联合保护作用中的应用

ThejointprotectiveeffectsofcopperandvitaminEagainstCCl4inducedhepaticdamage

利用两因素多水平析因试验设计，按Ｃｕ（０，０．０５，０．１，０．２，０．４ｍｇ／ｋｇ）和ＶＥ（０，１５０ｍｇ／ｋｇ）将６０只大鼠随机分成１０组，按组合条件饲养，７天后腹腔注射ＣＣｌ４，从血清酶学和组织酶学方面，探讨微量元素Ｃｕ和ＶＥ对ＣＣｌ４所致肝损伤的保护作用。结果表明：对ＳＯＤ、ＧＰＴ、ＧＯＴ３项指标单独Ｃｕ组和单独ＶＥ组与ＣＣｌ４组比较均有显著性差异（Ｐ＜０．０１），说明Ｃｕ和ＶＥ都对ＣＣｌ４所致肝损伤有保护作用。但对Ｃｕ来说，保护效果并不随剂量的增加而增强。同时，Ｃｕ和ＶＥ具有联合保护作用（Ｐ＜０．０１），当ＶＥ用量为１５０ｍｇ／ｋｇ时，Ｃｕ的用量为０．１ｍｇ／ｋｇ。

hispaperstudiedthejointprotectiveefectsofcopper(Cu)andvitaminE(VE)againstCCl4inducedhepaticdamageusingfactorialdesign.Sixtyratswererandomlydividedinto10groups(VE0,150mg/kg;Cu0,0.05,0.1,0.2and0.4mg/kg).TheratswerefedwithcombineddiferentCuandVEwithacombina-tiondosages.Sevendayslater,ratswereinjectedintraperitonealywithCCl4(230mg/kg).Theresultsindi┐catedthatthereweresignificantdifferencesintheactivitiesofserumGPTandGOTandliverSODamonggroupswithorwithoutVEandvariousCudosage(P<0.01).CuandVEhaveobviousprotectiveeffectsa┐gainstCCl4inducedhepaticdamage.TheprotectiveefectsofCuwerenotimprovedbytheincreaseofdosages.CuandVEhavejointprotectiveeffects(P<0.01).WithVE150mg/kgandCu0.1mg/kg,thebestprotectiveeffectswereobtained.