摘要
在0.5mol/LH2SO4介质中,Br-和Bro3-反应生成Br2。4',5'-二碘荧光素与Br2反应,使4',5'-二碘荧光素荧光猝灭,当加入苯酚时,苯酚的溴代反应使体系荧光增强,增强的荧光强度与苯酚溶液浓度在一定范围内成线性关系,由此建立了一种测定苯酚的荧光分析新体系。在pH4.0-6.0范围内,该体系激发波长,发射波长分别为520nm,545nm。苯酚浓度为1.2-55μg/L范围内呈线性关系,检测出限为1.2μg/L,本法用于苯酚的测定,选择性好,结果满意。
A new highly sensitive and selective fluorimetric method has been developed for the determination of trace amounts of phenols, with 4＇,-5＇-diiodo-fluorescein as the fluorescent reagent （λex = 520, λex = 545nm）. At pH 4.0 - 6.0. Br - reacts with Bro3 - in sulfuric acid solution （0.5mol/L） to produce bromine （Br2）, which then reacts with 4＇-5＇-diiodo-fluorescein resulting in fluorescence quenching. The fluorescence intensity of the solution increases with increasing concentration of phenols presented. The linear range is 1.2 - 55μg/L, and the detection limit is 1.2 μg/L. The results, have heen satisfactory.
出处
《福建分析测试》
CAS
2007年第1期12-14,共3页
Fujian Analysis & Testing
