三种从马血中提取基因组DNA方法的比较研究

Comparative Research on Three Methods of Genomic DNA Extracted from Horse Blood

选择一种快速、简便、经济的从马血液中提取基因组DNA的方法。分别采用了常规酚—氯仿抽提法、简易酚—氯仿快速抽提法和V—gene Biotechnology Limited试剂盒三种方法从马血中提取DNA,并利用琼脂糖凝胶电泳技术和紫外分光光度计,比较各方法提出DNA的浓度和纯度,且利用PCR扩增技术检测DNA的实用性。结果表明,常规酚—氯仿抽提法适合从马血中大量提取DNA。

To choose a fast and economic methods for genomic DNA extraction from horse blood, with routine phenol-chloroform method, simple rapid phenol-chloroform method and V-gene Biotechnology Limited kit, then use spectrophotometer to compare density and purification of DNA and utilize PCR ampliation technique to detect practicability of DNA. The result shows that routine phenol-chloroform method was adapt to extract a mass of DNA.