结肠腺癌组织p27^(kip1)表达水平及与临床病理因素的关系

The expression of p27^(kip1) in colorectal adenocarcinoma and the correlation with the clinicopathological features

目的观察结肠腺癌组织p27kip1表达水平,探索其与肿瘤分化程度等临床病理因素间的关系。方法HE和免疫组化染色,常规观察与图像分析结合测量结肠腺癌等组织p27kip1阳性细胞体积密度、阳性细胞比率,分析其与肿瘤分化程度等因素间关系。结果结肠腺癌组织癌细胞体积密度与对照肠粘膜上皮间无明显差别,但其p27kip1阳性表达率明显低于对照肠粘膜和结肠腺瘤组织,与邻癌粘膜组织差异无显著性。结肠腺癌p27kip1表达水平与肿瘤分化程度、浸润深度及淋巴结转移相关,与患者性别、年龄、肿瘤大小无关。结论结肠腺癌组织p27kip1表达水平降低,且与肿瘤恶性程度、侵袭能力、淋巴结转移有关,可作为判定结肠癌恶性程度及预后的一个指标。

Objective To investigate the expression of p27^kip1 protein in coloreetal adenocarcinoma and relationship with the biological behaviors. Methods p27^kip1 expression was detected by immunohistochemistry using two-step ways of En Vision system, The numberical densities and the positive rates of p27^kip1 were measured by image analysis. Results The expression of p27^kip1 was significantly lower in colorectal carcinoma tissue than in normal colorectal mucosa and adenoma, but it had not difference as compared with the adjacent mucous tissue, p27^kip1 expression in carcinoma tissue was correlated significantly with differentiation grade, invasive depth and local lymph node metastasis. Conclusion The decrease of p27^kip1 expression may be involved in the malignant transformation of colorectal epithelial cells to colorectal adenocarcinoma.