蛋白激酶C与微波辐照致学习记忆损伤的关系

RELATIONSHIP BETWEEN PKC AND MICROWAVE IRRADIATION INDUCED LEARNING AND MEMORY INPAIRMENT

[目的]探讨微波辐照对大鼠学习记忆的损伤与PKC信号传导途径的关系。[方法]以峰值功率密度为65 W/cm2的微波辐照大鼠20 min,用水迷宫检测大鼠学习记忆功能,用改良的Takai法检测海马PKC活性,用West-ern-blot法检测海马AMPA受体磷酸化程度。[结果]微波辐照后,大鼠寻找平台潜伏期由15.1 s增加到20.9 s(P﹤0.05);细胞膜上PKC活性降低,细胞质内PKC活性升高;辐照后0 h、3 h AMPA受体磷酸化水平下降,从6 h到24h,恢复至对照水平。[结论]峰值功率密度为65 W/cm2的微波辐照对学习记忆的损伤可能涉及PKC-AMPA途径的功能变化。

[Objective] To study the relationship between PKC signal transduction,pathway and microwave irradiation induced learning and memory impairment. [ Methods] After exposure to 65 W/cm^2 microwave, the spatial learning and memory ability was tested by Morris water maze method, the change of PKC kinase activity in rat hippocampus was detected by Takai method, AMPA receptor phosphorylation level was determined by Western-blot method. [ Results] After 65 W/cm^2 microwave exposure, deficit of learning and memory ability in vat was observed. 65 W/cm^2 microwave irradiation reduced PKC kinase activity in membrane, and suppressed AMPA receptor phosphrylation in rat hippocampus. [Conclusion] The results showed that PKC-AMPA pathway may take part in the learning and memory injury induced by 65 W/cm^2 microwave irradiation.