摘要
目的 建立简便的循环酶法,直接检测血浆同型半胱氨酸(Hcy)。方法 在Hitachi 7170S全自动生化分析仪上,以循环酶法测定血浆Hey,采用两点速率法,主波长340nm,副波长405nm,温度37℃,两点线性校准。结果 本法线性范围1.5—50μmol/L;日内不精密度为2.2%-3.0%,日间不精密度为4.0%-5.9%;回收率为94.6%-102.5%。循环酶法(Y)和商品试剂盒(X)比较:Y=1.018X-0.895,决定系数(r^2)=0.945。结论 循环酶法简便、灵敏,试剂稳定,适用于血浆Hcy的常规和自动化分析。
Objective To establish a simple enzymatic cycling assay for determining homocysteine (Hcy) in plasma. Methods The plasma Hcy was detected by enzymatic cycling method on Hitachi 7170S automatic analyzer. The determation parameter: 2-point rate assay, 2-point linear calibration, the temperature was 37 ℃, the main wavelength was 340 nm and the secondary wavelength was 405 nm. Results The linearity of this method was 1.50-50 μmol/L. The within-run coefficien of variation(CV) was 2.2% -3.0% , the between-run CV was 4.0% -5.9%. The recovery rate was 94.6%-102.5%. Correlation between results obtained by this method(Y) and commercial reagent assay(X) was Y = 1. 018X-0.895 ,decisive coefficien( r^2) =0.945. Conclusions The method is simple, sensitive and stable for routine and automatic measurement of Hey in plasma.
出处
《检验医学》
CAS
北大核心
2007年第1期16-19,共4页
Laboratory Medicine
基金
山东肥城矿业集团公司基金资助项目(20051003)