外伤后癫痫动物模型的建立

Model Establishment of Post-traumatic Epilepsy

目的将铁离子导入到大鼠的感觉运动皮质内,造成大鼠的外伤后癫痫的动物模型,观察大鼠术后癫痫发作的行为学改变。方法用离子导入法将铁离子导入到SD大鼠大脑皮质内,通电时间为10 min,通电电流为200μA。对照组大鼠给予相同的手术操作,但不导入铁离子。结果实验组内20只大鼠有18只出现癫痫发作,癫痫模型制作成功率为90%;对照组内20只大鼠内有一只出现癫痫发作,癫痫模型制作成功率为5%。结论用离子导入法制作大鼠外伤后癫痫动物模型的通电电流以200μA为最佳条件,通电时间为10 min。制造出来的模型的成功率较高,且为急性模型。

Objective To introduce iron ions into the sensory and motor cortex of rat, then get the rat model of posttraumatic epilepsy, to observe the behavioural changement when the postoperated rats exhibit epilepsy. Methods We anesthefized the experimental SD rats by injecting 2% pentobarbital sodium, 40 mg/kg into abdominal cavity, then introduced iron ions into the cerebral cortex of rats, 10 minutes with 200μA electric current. The control group was treated by same operation, but no ion introduction. Results 18 of 20 rats were seizures, the succeeded rate of rat model were 90% ; For the control groups, 1 of 20 were seizures, rate were 5%. Conclusion The appropriated conditions for the rat model of post-traumatic epilepsy with ion introduction are 200μA currency and 10 minutes given. It is acute-style model and has higher succeeded rate.