冠状动脉瘤组织中肺炎衣原体特异性抗原的表达

Expression of chlamydia pneumoniae specific antigen in human coronary artery atheroma tissues

目的探讨肺炎衣原体特异性抗原(Cpn-Ag)表达与冠状动脉瘤之间的关系。方法采用自制肺炎衣原体单克隆抗体的直接微量免疫荧光法检测50例冠状动脉瘤标本和20例正常冠状动脉标本中Cpn-Ag的表达。结果冠状动脉瘤和正常冠状动脉标本中Cpn-Ag的阳性率分别是58.0%(29/50)和15.0%(3/20)。冠状动脉瘤标本中有大量Cpn-Ag沉积,分别位于外膜下及粥样硬化斑块内,而正常血管壁中仅有少量Cpn-Ag沉积,两者具有统计学差异(P<0.01)。结论Cpn-Ag在冠状动脉瘤外膜下有高表达率,Cpn可能是参与冠状动脉粥样硬化形成损害的原因之一。

Purpose To explore the relationship between expression of chlamydia pneumoniae specific antigen（Cpn-Ag） and coronary artery atheroma. Methods The Cpn-Ag was detected by direct immunofluorescence using self-made monoclonal antibody of Cpn for 50 archival paraffin-embedded coronary artery atheroma specimens and 20 normal coronary artery specimens. Results Positive rates of Cpn-Ag in coronary artery atheroma specimens and normal artery specimens were 58.0% （29/50） and 15.0%（3/20）, respectively （P〈0.01）. A lot of apple-green spots located in atherosclerotic plaques and in adventitia of coronary artery atheroma specimens, but only a little spots were founded in adventitia of normal artery specimens. Conclusions The high expression rates of Cpn-Ag were founded in adventitia of coronary artery atheroma, the Cpn may be one of causational factors for the formation of lesions within the coronary artery atherosclerosis.