四份花培来源的水稻不育系种质的SSR分析

SSR Analysis of Four Rice Male Sterile Lines Derived from Rice Anther Culture

利用微卫星标记和靶区域扩增多态性(targe tregion amplification polymorphism,Trap)系列引物标记对本研究所选育4份不育系(S446A、S409A、S13A和S560A)材料进行指纹图谱构建,从294对SSR引物和8对Trap引物中筛选出22对呈多态性的引物,其中引物RM222、RM171、RM251和引物Trap2、Trap3能将花培不育系与其他8种不育系区分开,这就在DNA水平上找到它们的区别,利用这些引物对12份材料进行聚类分析表明,不育系的遗传基础均较狭窄。但我们也从DNA水平找到差异,说明分子标记鉴定结果的准确性,可用于新材料的成果认定和专利获得。

The four rice male sterile lines derived from anther culture were identified using 294 SSR primers pairs and 8 Trap （target region amplification polymorphism） primers pairs. 22 primers pairs had polymorphism among these male sterile lines. SSR primers, RM222, RM171 and RM251 and Trap primers, Trap 2 and Trap 3, can distinguish between four male sterile lines and other 8 male sterile lines that are widely released in China so far. Dendrogram of similarity indicated that the genetic diversity among these 12 male sterile lines were quite narrow. But the different at DNA level still could be found easily, which indicated that SSR markers could be used in indentification of new germplasms in rice .