香椿叶片组织培养和快繁技术的研究

Studies on Leaf Tissue Culture and Rapid Propagation of Toona sinensis

研究了香椿叶片组织培养以及无菌苗的移栽技术。结果表明,MS+1.0 mg.L-1 BA+0.1 mg.L-1 KT+0.5 mg.L-1 NAA培养基对香椿叶片愈伤组织诱导的效果最佳,诱导率高达100%,诱导时间最短,仅为6d;MS+1.5 mg.L-1 GA3+1.0 mg.L-1 BA+0.5 mg.L-1 KT对香椿叶片分化出来的幼芽增殖效果最好,其增殖率高达63%;1/2MS+1.5 mg.L-1 IBA+30g蔗糖作为生根培养基最佳,生根率达到了89%。

The leaf tissue culture and transplant techniques of Toona sinensis we＇re studied. The results showed that the MS culture medium with 1.0mg·L^-1 BA, 0.1mg·L^-1 KT and 0.5mg·L^-1 NAA was the best for the inducing leaf callus of Toona sinensis. The leaf inducement rate was 100% and the inducement time was six days. The MS culture medium with 1.5mg·L^-1 GA3, 1.0mg·L^-1 BA and 0.5mg·L^-1 KT was the best for the leaf multiplication, the leaf multiplication rate was approximately 63%. The root growth culture medium with 1/2MS, 1.5mg·L^-1IBA, and 30g cane sugar was optimum, and the root growth rate was 89%.