汉坦病毒Vero细胞灭活疫苗候选毒种筛选

Selection of virus strain for production of inactivated hantavirus vaccine of vero cells

目的将肾综合征出血热疫苗后备筛选毒株适应于Vero细胞，并对其抗原性和免疫原性进行研究。方法将新分离的4株汉坦病毒接种敏感动物，在乳沙鼠脑内连续传代。比较脑内病毒抗原的含量以及乳鼠的发病情况；在Vero细胞中传代，比较培养液中的病毒滴度和抗原滴度以及细胞的病变情况；利用筛选出的毒株研制Veto细胞灭活疫苗。研究疫苗的免疫原性。结果4株病毒经乳沙鼠脑内传代，鼠脑悬液的病毒滴度和抗原滴度分别达7．00～7．75LgCCID50／ml和1：3201：1280；4株毒株经Vero细胞连续传5代，培养液病毒滴度和抗原滴度分别达6．50～7．50LgCID50／ml和1：160～1：768；疫苗免疫家兔2针后，其中出血热病毒株Z34与ZJ5株疫苗免疫血清对同型毒株的中和效价达到1：10。结论ZJ4与ZJ5两毒株已适应于Vero细胞，且具有病毒滴度高和免疫原性良好的特性，适合用作Vero细胞肾综合征出血热疫苗理想的候选毒株。

Objective To adapt the candidate strains of hemorrhagic fever with renal syndtome（HFRS）vaccine to Veto cells and to study their antigenicity and immunogenicity. Methods 4 strains of Hantavirus were continuously propagated in suckling mouse brains or in Veto cells and studied the antigenicity of virus, viral titers, the amounts of virus antigen and their immunogenicity. Results Four strains of Hantavirns adapted to Veto cells and stably grew in the cells or in suckling mouse brains with high titers. Rabbits immunized with the crude vaccines of ZJ4 and ZJ5 showed the neutralizing antibody titers of the rabbit immune sera reached 1 ： 10 at 4 weeks after 2 times of immunization. Conclusion ZJ4 and ZJ5 candidate strains adapted to Vero cells, possessed high titers with good immunogenicity and were feasible to prepare the HFRS vaccine of Veto cells.