连花清瘟胶囊体外抗甲型流感病毒的实验研究

Anti-viral Effects of Lianhua Qingwen Capsule Against Influenza A Virus in Vitro

目的研究连花清瘟胶囊体外抗甲型流感病毒的作用。方法以利巴韦林作阳性对照药物,采用存留细胞结晶紫染色法测定连花清瘟胶囊不同给药方式对甲型人流感病毒H3N2的体外抑制作用及其时效关系。结果连花清瘟胶囊具有多环节抗甲型人流感病毒的作用:综合抑制作用、对病毒吸附的预防作用、抑制病毒吸附后的复制增殖作用以及直接杀伤病毒的作用,这4种作用的半数有效浓度(EC50)分别为0.042,0.031,0.051,0.050g/mL,以预防给药方式抗流感病毒的作用最强。随药物作用时间的延长,连花清瘟胶囊的低浓度抗病毒有效率呈减少趋势,在高浓度时(≥0.031g/mL)其抗病毒能力基本不变。同时连花清瘟胶囊可明显降低病毒的感染性。结论连花清瘟胶囊具有明显的体外抗甲型人流感病毒作用。

Objective To study the anti- viral effects of Lianhua Qingwen Capsule against influenza A virus in vitro. Methods Under different administration modes, the inhibitory effect of Lianhua Qingwen Capsule on human influenza A virus （H3N2） in vitro and its time -effect relationship were assayed by crystal violet staining assay with ribavirin as positive reference drug. Results Lianhua Qingwen Capsule showed a multi- access effect on anti- human influenza A virus, such as comprehensive inhibition effect, prevention effect on virus adsorption, inhibition on virus proliferation after adsorption and direct killing the virus effect, and the median effective concentrations （EC50） in above -mentioned effects were 0. 042, 0. 031, 0. 051 and 0. 050 mg/mL respectively. Among them, the prevention effect on virus adsorption was the strongest . With the expanding actuation duration of the drug, the inhibitory effect of Lianhua Qingwen Capsule against influenza A virus weakened at low concentration, but no change at high concentration （ ≥ 0. 031 g/mL）. Meanwhile, Lianhua Qingwen Capsule can remarkably decrease the infectivity of influenza A virus. Conclusion Lianhua Qingwen Capsule has an obvious effect against human influenza A virus in vitro.