内蒙古产甘草药材HPLC指纹图谱研究

Study on the HPLC Fingerprints of Glycyrrhiza uralensis Fisch.Produced in Inner Mongolia

目的研究乌拉尔甘草(Glycyrrhiza uralensis Fisch.)药材的指纹图谱测定方法,建立特定产地甘草药材的HPLC标准指纹图谱。方法采用反相高效液相色谱法,选用PhenomenexC18柱(4.6mm×250mm,5μm),乙腈-醋酸盐缓冲溶液为流动相梯度洗脱,运用梯度波长采集法,采集时间为60min,流速为1mL/min。结果建立甘草药材的HPLC指纹图谱,标定了甘草药材10个共有指纹峰,方法学考察结果符合指纹图谱技术要求。结论该方法稳定、准确、可靠,为甘草药材质控标准的制定提供了科学依据。

Objective To develop a HPLC fingerprints of Glycyrrhiza uralensis Fisch. in Inner Mongolia. Methods The RP - HPLC method was adopted on Phenomenex C18 （4. 6 × 250mm, 5 μm） analytical column by gradient elution with acetonitrile and acetic acid buffer solution as mobile phase. The gradient wavelength data acquisition was employed. The analysis time was 60 minutes and the flow rate was 1 mL/min. Results The HPLC standard fingerprints chromatography of Glycyrrhiza uralensis Fisch. consists of 10 common characteristic peaks. The results of method validation accorded with the technical standard of fingerprints. Conclusion This method is stable, accurate, reliable, and can provide a scientific basis for the quality control of Glycyrrhiza uralensis Fisch.