摘要
目的:利用高效液相色谱法分离、测定六味地黄汤中梓醇,完善质量控制方法。方法:用kromasil ODS-1柱(200mm×4.6mm)色谱柱,0.5%的乙腈溶液为流动相,检测波长为210nm,柱温25℃。结果:梓醇在0.0673。1.346μg范围内呈艮好的线性关系,平均回收率为97.95%,RSD=0.96%(n=5)。结论:该法方便简便、快速,准确可靠。
Objective: In order to improve the method of quality control, the HPLC was used to separate and determine the catalpol in Rehmanniae decoction of six ingredients. Method: The chromatographic conditions included the kromasil ODS- 1 column (200 mm ×4.6 mm) and mobile phase consisted of 0.5% acetonitrile, the detection wavelength was 210 nm. Result: The catalpol showed a good linear relationship at the range of 0. 0673 × 1. 346μg. the average recovery was 97.75% with RSD 0.96%. Conclusion: The method is simple, fast, accurate and reliable.
出处
《中医药导报》
2007年第1期62-63,共2页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
关键词
高效液相色谱法
梓醇
六味地黄汤
质量控制
HPLC
Catalpol
Rehmanniae Decoction of Six Ingredients
Quality control