芡实的化学成分

Chemical Constituents of the Seeds of Euryale ferox

目的：研究睡莲科芡属植物芡（Euryale ferox Salisb．）的成熟种仁芡实的化学成分，为阐明其有效成分提供科学依据。方法：用95％甲醇回流提取，通过硅胶、Pharmadex LH-20凝胶、RP-18反相柱层析进行分离，利用化合物的理化性质和光谱数据鉴定其结构。结果：从中分离鉴定了7个化合物和1个含有2个化合物的混合物，它们的结构分别为：环（脯-丝）（1）、环（异亮-丙）（2）、环（亮-丙）（3）、5，7，4′-三羟基-二氯黄酮（4）、5，7，3′，4′，5′-五羟基二氢黄酮（5）、异落叶松树脂醇-9-O-β-D-吡喃葡萄糖苷（6）、α-生育酚（7）、β-生育酚（8）和昏生育酚（9）。结论：化合物1为首次从自然界分得，化合物2—6为首次从该植物中分离得到。

AIM： To investigate the chemical constituents in the seeds of Euryale ferox Salisb for obtaining a more comprehensive understanding on its effective components. METHODS： Compounds were separated by column chromatography with silica gel, Phannadex LH-20 and reversed-phase silica gel. Their structures were elucidated by spectral analysis and chemical evidence. RESULTS： Seven compounds and a mixture of two compounds were obtained. Their structures were identified as： Cyclo（Pro-Ser ） （1）, Cyclo（Ile-Ala） （2）, Cyclo （keu-Ma） （3）, 5, 7, 4＇-trihydroxy-flavanone （4）, 5, 7, 3＇, 4＇, 5＇-pentahydroxy-flavanone （5）, isolariciresinol-9-O-β-D- glucopyranoside （6）,α-tocopheml （7）, β-tocopheml （8）, δ-tocopherol （9）. CONCLUSION： Compound 1 was firstly isolated from natural sources; compounds 2-6 have been isolated from this plant for the first time.