膜技术对肉毒发酵液纯化的中试应用研究

The middle scale application of membrane technology for purification of botulin fermentation broth

报道了把膜分离技术应用于肉毒发酵液的纯化．用孔径为０．６５μｍ的微滤膜对发酵液进行除菌，除菌率达到１００％；再以超滤法对毒素溶液进行浓缩，当毒素液被浓缩１０倍后，其毒力效价达到未浓缩毒素溶液毒力效价的４８倍．并对分离过程的动力学进行了初步研究．把该技术进一步放大到中试规模的应用，获得了较好的结果，对发酵液的处理量可达５０ｋｇ／ｈ．

Botulinus toxin from a clostridium botulin fermentation broth was separated and purified by membrane filtration technology. To remove the bacteria from the fermentation broth, we chose a 0.65 μm pore size microfiltration membrane, reaching a removal ratio of 100%. In the second experiment, we used ultrafiltration to concentrate the toxin solution by 10 times, resulting in 48 fold increase of toxin efficacy. In a middle scale installasion, we were able to process about 50 kg/h of botulin solution.