广西肝癌高发区壮族自然人群中流行的HCV、HBV的分子生物学研究

Molecular biological research of hcv and hbv found in natural population of zhuang people in a hcc high incidence area of Guangxi

在广西肝癌高发区壮族自然人群中采集血清标本１７７６人份，采用ＥＬＩＳＡ方法检测抗－ＨＣＶ、ＨＢｓＡｇ，ＰＣＲ技术检测ＨＣＶ－ＲＮＡ、ＨＢＶ－ＤＮＡ，并对ＨＣＶ－ＲＮＡ阳性的６９份标本采用型特异性引物进行基因分型。结果显示：（１）研究人群中ＨＣＶ－ＲＮＡ阳性率为３．８８％；（２）流行的ＨＣＶ基因以Ⅰ型或与其它型别的混合感染为主；（３）Ⅰ型感染者中ＡＬＴ的异常率高于其它型别的感染者；（４）当地ＨＣＶ伴随感染ＨＢＶ的现象比较常见。上述发现是否与当地肝癌高发有关。

serum samples were obtained from inhabitants of zhuang nationality lived in a HCC high incidence area in GuangXi Antibodies to hepatitis C virus and HBsAg were detected with a enzyme immunoassay;The HCV-RNA and HBV-DNA were detected by polymerase chain reaction;Genotyped using type-specific primers on 69 samples that HCV-RNA were positive The results showed that:(1)the HCV prevalence rate was 3 88%(69/1776),(2)type I HCV and mixed with other types were predominant in the studied population,(3)the abnormal rate of ALT among peoples infected with type I HCV was much more than that of infected with other types;(4)The HCV infection mixed with HBV was prevailing in the population It was suggested that the high incidence of HCC in the surveyed area might associate with the factors as indicated above