蒙古种沙土鼠背海马胆碱能投射来源

ORIGINS OF ACETYLCHOLINESTERASE-CONTAINING PROJECTIONS IN DORSAL HIPPOCAMPUS OF MONGOLIAN GERBIL

应用HRP逆行追踪结合乙酰胆碱醋酶(Acetylcholinesterase AchE)组化技术,研究了25例蒙古种沙土鼠背海马的胆碱能投射来源.将HRP引入背海马后行AchE-HRP联合反应,在同侧内侧隔核、斜角带核、视前大细胞核等处(基底大细胞核)出现AchE-HRP双标记神经元胞体,并在背海马局部发现广泛存在着AchE纤维和末梢.而未见强染色的AchE阳性胞体.以上结果提示:沙土鼠背海马的乙酰胆碱主要是外源性的,其胆碱能传入主要来自基底前脑区的胆碱能神经元.基底前脑-海马的胆碱能通路的存在为研究学习、记忆功能和老年性痴呆的发生机理提供了一定的形态学依据.

By means of HRP retrograde tracing method combined with histochemical AchE method, the origins of AchE-containing projections in the dorsal hippocampus of mongolian gerbil were studied. After the injection of HRP solution into the dorsal hippocampus followed by the procedure for simultaneous demonstration of HRP and AchE, the double labelled neurons containing the both reactive products of HRP and AchE were seen in the ipsilateral nuclei of the diagonal band. medial septal nuclei and preoptical magnocellular nuclei (NBM magnocellular basal nucleus).A lot of AchE fibers and terminals were discovered but no densely-stained AchE neuron was seen in the local dorsal hippocampus. The result showed that the cholinergic afferents of the dorsal hippocampus were mainly derived from the cholinergic neurons located in the NBM. The significance of the cholinergic path of the basal forebrain-hippocampus is discussed.