大鼠背根切断后脊髓胶状质内鸟核苷酸调节蛋白的改变

CHANGE OF GUANINE NUCLEOTIDE-REGULATING PROTEIN IN SUBSTANTIA GELATINOSA OF SPINAL CORD OF RATS AFTER UNILATERAL TRANSECTION OF DORSAL ROOTS

用兔抗鸟核苷酸调节蛋白α_0亚单位多克隆抗血清,采用免疫组织化学方法来观察大鼠脊髓内α_0.免疫反应性.对鸟核苷酸调节蛋白进行定位.及其在切断脊神经背根(简称背根)后的改变.结果发现:正常鼠和对照组鼠脊髓后角浅层均出现强的α_0,免疫反应性,第二层(胶状质)最为密集,外侧脊核区也出现α_0免疫反应性纤维网.切断背根后肢状质内α_0免疫反应性及其光密度定量均明显降低,表明胶状质内与一级伤害性刺激传入有关的神经元末梢内鸟核苷酸凋节蛋白部分来源于一级感觉神经元,提示鸟核苷酸调节蛋白可能参与调控一级感觉的传递.

The subunit a,, of guanine nucleotide-binding protein (rabbit polyclonal antiserum 9120, K. J. Chang, USA) was demonstrated in the areas of rat spinal cord by immunohistochemical PAP methods, and the changes of α0-immunoreactivity(α0-IR) in the substantia gelatinosa(SG) were observed after unilateral transection of dorsal roots. In the normal and control animal intense a0-IR was presented in Rexed lamina 1 to III of the dorsal horn, and the highest α0-IR in lamina SG. Lateral spinal nucleus of rat revealed higher density of α0-IR containing fiber networks. Follwoing unilateral transection of dorsal roots, α0-IR(observation under microscope, optic density) was markedly decreased as compared with the normal and control group in those areas. The results indicated that Go-protein in the terminals of the primary nociceptive neurons of SG decreased markedly after dorsal roots were transected. It was inferred that partial Go-protein probably originated from the primary sensory neurons. This study suggests that Go may play an important role in the primary afferents informational regulation.