摘要
Solid-phase extraction method has proven efficient and amenable to microchips.A novel DNA purification microchip was demonstrated using porous silicon dioxide as the solid phase matrix.Porous SiO_2 was coated on the wall of the channel of the microchip to provide a surface-enlarging matrix.The porous silicon was achieved by anodisation of the silicon reactor in a HF/ethanol mixture and then was oxidized thermally at a lower increasing/decreasing rate to form porous SiO_2.Through optimization of the preparing conditions a more stable microchip was established.Compared with the microchip without the porous layer,the microchip with the porous SiO_2 layer achieved higher extracted efficiency of DNA.Genomic DNA from rat whole blood could be extracted and purified in 35 minutes.
Solid-phase extraction method has proven efficient and amenable to microchips. A novel DNA purification microchip was demonstrated using porous silicon dioxide as the solid phase matrix. Porous SiO2 was coated on the wall of the channel of the microchip to provide a surface-enlarging matrix. The porous silicon was achieved by anodisation of the silicon reactor in a HF/ethanol mixture and then was oxidized thermally at a lower increasing/decreasing rate to form porous SiO2. Through optimization of the preparing conditions a more stable microchip was established. Compared with the microchip without the porous layer, the microchip with the porous SiO2 layer achieved higher extracted efficiency of DNA. Genomic DNA from rat whole blood could be extracted and purified in 35 minutes.
出处
《稀有金属材料与工程》
SCIE
EI
CAS
CSCD
北大核心
2006年第A03期284-287,共4页
Rare Metal Materials and Engineering
基金
The authors greatly acknowledge the financial support from the National Science Foundation of China under Grant number 20299030, 60427001 and 60501020.
