The Construct of A Neutral Medium Applicable Electrochemiluminescent Sensor Based on the Chemical Modification of Cysteine and Luminol

In the phosphate buffer solution of pH＞7,the cysteine sensitized the electrochemiluminescence (ECL) of luminol.It could be modified on the surface of platinum electrode and furthermore adsorbing the luminol on its exterior to construct an ECL sensor.The ECL intensity of this sensor was strong enough and very stable.There wasn't obvious decrease of ECL intensity for thirty times of using in 48 hours with the relative standard deviation (RSD) of 0.98%.It could be used to determine some quenching effective molecules such as superoxide dismutase (SOD) with negative response upon the concentration range from 4.8 IU/ml to 57.6 IU/ml.

In the phosphate buffer solution of pH〉7, the eysteine sensitized the eleetroehemilumineseenee （ECL） of luminol. It could be modified on the surface of platinum electrode and furthermore adsorbing the luminol on its exterior to construct an ECL sensor. The ECL intensity of this sensor was strong enough and very stable. There wasn＇t obvious decrease of ECL intensity for thirty times of using in 48 hours with the relative standard deviation （RSD） of 0.98%. It could be used to determine some quenching effective molecules such as superoxide dismutase （SOD） with negative response upon the concentration range from 4.8 IU/ml to 57.6 IU/ml.