抗血管内皮生长因子抗体对人浆液性卵巢癌裸鼠移植瘤生长的影响

Effect of anti-vascular endothelial growth factor antibody on the progression of human serous ovarian carcinoma xenotransplanted into nude mice

目的研究抗血管内皮生长因子抗体(抗VEGF抗体)对浆液性卵巢癌生长的抑制作用。方法首次采用人浆液性卵巢癌组织直接接种而建立的裸鼠皮下移植瘤动物模型,观察有中和活性的抗人VEGF121单克隆抗体对肿瘤生长的影响。实验用BALB裸鼠分为两组,每组各6只,治疗组给予抗VEGF 121抗体,100μg/次;对照组给予等体积的磷酸盐缓冲液,均为腹腔内注射,自肿瘤接种后24 h起,每周2次,共9次;肿瘤接种一周起每次给药前测量肿瘤的长径及短径,并计算各时点体积;治疗结束后24 h处死动物,测量肿瘤的重量及计数肿瘤组织中的微血管密度(MVD)。结果①抗体治疗组与对照组裸鼠的体积逐渐出现差异,这种差异随时间的推移逐渐增大,接种第22天差异有显著性(P<0.05),并持续至实验结束。②用药结束后24 h处死裸鼠,治疗组平均瘤重为(0.70±0.11)g,对照组平均瘤重为(1.19±0.18)g,两组相比较有显著性差异(P<0.01)。③对照组肿瘤组织中微血管较密集,MVD值为7.60±1.14,治疗组肿瘤组织中的微血管明显减少,MVD值为4.80±0.83,两组相比较差异有显著性(P<0.05)。结论抗VEGF121单克隆抗体能够有效抑制裸鼠体内浆液性卵巢癌皮下移植肿瘤的血管形成,从而达到抑制肿瘤生长的目的。

Objective To study the inhibiting effect of the anti-VEGF121 monoclonal antibody on the progression of serous ovarian careinorna in subcutaneous xenotransplanted nude mouse model. Methods The tissues of human serous ovarian carcinoma were directly transplanted into nude mice to construct the model for studying the angiogenesis-inhibiting effect. Nude mice were randomly divided into two groups： control group with PBS, and treated group with anti-VEGF antibody. After xenotransplantation, they were given i. p, twiee a week for 9 times, The tumor volume and weight were evaluated after 1-week xenotrasnsplantation. The microvessel density （MVD） in tumor was immunohistochemically evaluated after treatment. Results There was significant differences in volume and weight of tumors between the two groups. The volume was smaller and weight was lighter in treated group than in the control group. MVD was significantly lower in treated groups than in control group. Conclusion Anti-VEGF121 antibody can inhibit angiogenesis of subcutaneously xenotransplanted tumor of human serous ovarian carcinoma into nude mice to inhibit its growth.