应激大鼠心肌线粒体三种氧化酶活性变化及β受体阻滞剂对其的影响

Activity alteration of three kinds of oxidase in myocardial mitochondria in stressed rats and the effects of β acceptor blocking agent on them

目的：进一步研究应激所致的心肌能量代谢变化及造成心肌损伤的机制．方法：将大鼠放置温水［（２０±１）℃］浸泡应激．在应激０．５，１，２，４，６ｈ后采用差速离心法分离线粒体，用氧电极法测定ＮＡＤＨ氧化酶、琥珀酸氧化酶、细胞色素Ｃ氧化酶的活性及其变化和运用β受体阻滞剂对其的影响．结果：不同应激状态下氧化酶的活性与正常组比较相差显著，在应激开始阶段活性增高而后期逐渐下降．运用β受体阻滞剂后其变化趋势无变化，但使应激后氧化酶活性下降时间向后推移．结论：应激后线粒体呼吸功能变化有一定规律．应用β受体阻滞剂不能阻断其变化过程．

Objective: To further study the alteration of myocardial energy metabolism and the mechanism of myocardial injuries under stressed conditions. Methods: The SD rats were put into warm water [(20±1)℃] for stress. After respective stress time of 0.5, 1, 2, 4, 6 h. The method of differential centrifugation was adopted to isolate mitochondria and the method of Clark' electrode used to measure the oxygen consumption of NADH, Suc, cytc oxidase. The activity of the oxidase was also calculated. The effects of β acceptor blocking agent were studied. Results: The activity of three kinds of oxidase of different stress time, rising over the beginning period of the stress and declining over the later period, were statistically significant compared on those of normal groups. Conclusions: Changes in the function of mitochondria in myocardiocyte for stress was regularity and the use the β acceptor blocking agent didn't bring any changee to the variation trend.