一氧化氮在大鼠梗阻性黄疸肝损伤中的作用

Experimental study on effects of NO on hepatic injury of rats with obstructive jaundice

目的：探讨一氧化氮（ＮＯ）在大鼠梗阻性黄疸肝损伤中的作用．方法：采用双重结扎、切断大鼠胆总管造成梗阻性黄疸模型．分别给不同组大鼠腹腔注Ｌ－精氨酸（Ｌ－Ａｒｇ）或Ｌ－精氨酸甲酯（Ｌ－ＮＡＭＥ），７ｄ后观察肝组织匀浆ＮＯ－２，丙二醛（ＭＤＡ），肝功能及肝组织形态改变．结果：胆道梗阻一周后，肝组织ＮＯ－２和ＭＤＡ含量均升高；注射Ｌ－Ａｒｇ［３００ｍｇ／（ｋｇ·ｄ）］组大鼠肝组织ＮＯ－２含量较单纯胆道梗阻组增加显著（Ｐ＜０．０１），而肝组织ＭＤＡ和血清ＡＬＴ，ＡＳＴ活性显著降低（Ｐ＜０．０５），肝组织损伤减轻；注射Ｌ－ＮＡＭＥ［７０ｍｇ／（ｋｇ·ｄ）］组大鼠肝组织ＮＯ－２含量下降（Ｐ＜０．０１），而肝组织ＭＤＡ含量和血清ＡＬＴ，ＡＳＴ活性却显著增加（Ｐ＜０．０１），肝组织损伤加重．结论：ＮＯ在大鼠梗阻性黄疸肝损伤中似有保护作用．

Objective: To investigate the effects of nitric oxide (NO) on hepatic injury of rats with obstructive jaundice. Methods: Rats were injected intraperitoneally with L Arginine [300 mg/(kg·d)] or L N G nitroarginine methyl ester (L NAME) [70 mg/(kg·d)] for 7 d after bile duct ligation (BDL). The levels of NO - 2, MDA in the liver and serum transaminase were measured. The morphological changes in the liver were also observed. Results: The levels of NO - 2 and MDA in the liver were elevated at 7 d after BDL. In BDL rats treated with L Arginine, the level of NO - 2 in the liver increased significantly, whereas the levels of MDA in the liver and serum transaminase decreased compared with those of BDL rats ( P <0.05) and the liver injury was lessened; In BDL rats treated with L NAME, the level of NO - 2 in the liver decreased, but the MDA level and serum transaminase activities increased compared with those of BDL rats ( P <0.01), and the liver injury was aggravated. Conclusion: NO may play a protective role in liver injury resulted from obstructive jaundice.