^（99）Tc^m标记白细胞在家兔脓肿和炎症患者显像中的初步应用

99 Tc m WBC preparation for inflammation imaging and preliminary application

目的：改良９９Ｔｃｍ－ＷＢＣ制备条件并评价其特性．方法：取１０ｍｌ家兔及人全血分离ＷＢＣ，用９９Ｔｃｍ－ＨＭＰＡＯ介导标记，评价ＷＢＣ分离率、９９Ｔｃｍ－ＷＢＣ的标记率、稳定性、活性及其在家兔脓肿模型和炎性肠病患者体内的炎症定位特性．结果：ＷＢＣ分离率达７０％，９９Ｔｃｍ－ＷＢＣ标记率和活性率分别高达６０％和９０％．放射性不断自ＷＢＣ中逸脱．注射９９Ｔｃｍ－ＷＢＣ后１０ｍｉｎ家兔大腿脓肿即显影，４ｈ时最清晰．９９Ｔｃｍ－ＷＢＣ可清晰地显示活动性炎性肠病受累肠段部位和范围．结论：１０ｍｌ血可分离和制备出满足临床显像要求的９９Ｔｃｍ－ＷＢＣ．

Objective: To improve 99 Tc m WBC preparation conditions and assess its properties for focal inflammation detection. Methods: Rabbit and human WBC were separated from 10 ml whole blood and labeled with 99 Tc musing 99 Tc m HMPAO. 99 Tc m WBC was evaluated in labeling efficiency, stability and viability. The property of 99 Tc m WBC in focal inflammation localization was evaluated in a rabbit abscess model and three patients with active inflammatory bowel disease (IBD). Results: The separation yield reach up to 70%, and 99 Tc m WBC labeling efficiency and viability were 60% and 90% respectively. Radioactivity eluted continuously from 99 Tc m WBC when in vitro incubated. 99 Tc m WBC accumulated in the rabbit abscess as early as 10 min post injection (p.i.) and culminated at 4 h p.i.. 99 Tc m WBC could definitely elucidated the inflammatory foci in patients with active IBD. No sideeffects were observed p.i. of the radiopharmaceutical. Conclusion: 10 ml whole blood could prepared desirable 99 Tc m WBC for inflammation detection.