摘要
分析T细胞信号传导分子TCRζ链基因在B细胞-急性淋巴细胞白血病(B-ALL)病人外周血中的表达水平。方法:采用SYBR G reenⅠ荧光定量PCR和相对定量检测15例初发B-ALL、5例完全缓解期(CR)患者和30例正常人外周血的单个核细胞的TCRζ链表达情况,以β2微球蛋白基因(β2M)作为内参,根据相对定量公式(2-ΔΔC t)分别计算B-ALL初发患者、CR期患者与正常人TCRζ链表达差异倍数等。结果:根据相对定量公式分析显示:与正常人相比,B-ALL初发患者外周血TCRζ链表达存在3种情况,即不表达(20%)、表达下降(53%)和表达上升(27%)。TCRζ链表达水平在所有初发B-ALL病人均低于CR期病人。结论:本研究首先报道B-ALL病人中TCRζ链表达水平的变化特点,TCRζ链基因表达水平以降低为主,可能与T细胞免疫缺陷有一定关系。
Objective : To analyze the expression level of signal transduction factor T lymphocyte-TCRξ chain gene in patients with B-acute lymphoblastic leukemia (B-ALL). Methods:Real-Time PCR with SYBR Green Ⅰ technique was used for detecting TCRξ chain expression level in peripheral blood mononuclear cells from 15 patients with untreated de novo B-ALL, 5 patients with B-ALL completely remission (CR) and 30 normal individuals. β2 - microglobulin gene ( 132 M) was used as an endogenous reference. Relative changes in TCRξ chain expression levels was used by the 2^△△Ct method among patients with B-ALL, CR patients, and normal individuals. Results: The expression level of TCRξ chain in patients with B-ALL could be divided into absent expression ( 20% ), down expression (53%) and over expression (27%) groups. The expression level of TCRξ chain in all de novo B-ALL patients was lower than that in CR patients. Conclusions: It is to our knowledge, the first description in the expression feature of TCRξ chain in B-ALL. The expression level of TCRξ chain was down expression in the most of B-ALL patients, and might relate to cellular immuneodeficiency.
出处
《广州医学院学报》
2006年第4期22-24,共3页
Academic Journal of Guangzhou Medical College
基金
国家自然科学基金项目(30270579
30424003)
广东省自然科学基金重点项目(23001)
血液病学国务院侨办重点学科建设基金资助(编号)