火把花根防治同种移植肾慢性排斥反应实验研究

Preventive Effect of Tripterygium Hypoglaucum on Chronic Allograft Nephropathy in Rats

目的:探索更有效、安全和经济的防治移植肾慢性排斥反应的途径,为临床提高远期移植器官存活率提供理论和技术基础。方法:应用标准的慢性排斥反应动物模型,观察移植24周后135mg/d火把花根片灌胃组和单纯肾移植对照组间24h尿蛋白定量、血肌酐和组织病理改变等的差异,评价火把花根对移植肾功能的影响。结果:火把花根可减少慢性排斥反应大鼠的尿蛋白、血清肌酐以及移植肾单个核细胞浸润和慢性纤维化病理评分,与对照组相比差异有显著意义(P<0.05)。结论:火把花根可有效减轻大鼠慢性排斥反应病变程度,可为临床防治慢性移植肾病提供新的措施和更广泛的选择。

Objective：To explore an effective, safe and inexpensive approach for prevention of chronic allograft nephropathy which would form a guidance for improved long-term survival of allografts. Methods： Rat models of chronic allograft nephropathy were divided into the tripterygium hypoglaucum group and control group. Rats in tripterygium hypoglaucum group were given 135 mg/d of the trial drug during the experiment. Twenty-four weeks after kidney transplantation,the changes of 24 h urine protein, serum creatinine and histology between the groups were compared. Results：The 24 h urine protein, serum creatinine, and pathological grades of monocyte infiltration and fibrosis in allografts in tripterygium hypoglaucum group were less severe than those in control group. Conclusion： Tripterygium hypoglaucum might attenuate chronic allograft nephropathy,which offered a cheaper choice for the treatment of chronic allograft nephropathy.