潘生丁对大鼠移植肝缺血再灌注损伤保护作用的实验研究

Experimental Studies on the Protective Effect of Dipyridamole Against Hepatic Ischemia-reperfusion Injury in Rat Liver Transplantation

目的探讨潘生丁对大鼠移植肝缺血再灌注损伤的保护作用及其相关机制。方法采用改良Kam ada“二袖套法”制作肝移植模型,48只雄性SD大鼠随机分成3组:假手术组(A组),同基因大鼠肝移植组(B组)和潘生丁预处理+同基因大鼠肝移植组(C组)。于供肝再灌注2 h后测定各组PAGT、ALT、AST、SOD、MDA含量,比较肝细胞凋亡和组织形态学改变。结果移植肝再灌注2 h后C组较B组肝组织损害轻,SOD、MDA、ALT、AST、PAGT变化及肝细胞凋亡差异有显著性(P<0.05)。结论潘生丁可通过改善肝脏微循环状况,改善缺血肝脏组织的能量代谢,提高肝组织抗氧化能力,抑制细胞凋亡和减少肝脏细胞的变性坏死程度而对肝脏热缺血再灌注起保护作用。

Objective To investigate the protective effect and its mechanism of dipyridamole against hepatic ischemia-reperfusion injury in rat liver transplantation. Method An improved Kamada＇s two-cuff technique was used for the liver transplantation. 48 male SD rats were randomly divided into 3 groups, sham operation （A）, allograft liver transplant （B） and dipyridamole preconditioned allograft liver transplant （C）. The tissue concentration of PAGT, ALT, AST, SOD, MDA were measured 2 hours after reperfusion, the apoptosis of liver cells and morphological change were also studied. Result There were significant differences between group C and B with regard to PAGT, ALT, AST, SOD, MDA and apoptosis of liver cells（ P 〈 0.05）. Conclusion The protective effect of dipyridamole against hepatic warm ischemia-reperfusion injury can be achieved through improvement of microcirctdation, energy metabolism, antioxidation, antiapoptosis and reduction of hepatic cell degeneration.