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腺相关病毒载体的制备及其在耳蜗转基因研究中的应用 被引量：1

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摘要 耳蜗基因治疗已成为人们积极探索的耳聋治疗方法之一,病毒载体的应用使耳蜗基因治疗取得了进展。腺相关病毒(adeno-associatedvirus,AAV)作为新兴的基因治疗载体在耳蜗基因治疗研究中具有广泛的应用前景。本文就AAV载体的制备及检测方法,AAV载体各血清型对耳蜗内各种细胞的转导率,AAV载体的安全性以及AAV载体转导目的基因在耳蜗基因治疗研究中的应用进行综述。
作者 刘玉和
机构地区 北京大学第一医院耳鼻咽喉头颈外科
出处 《中华耳科学杂志》 CSCD 2006年第4期343-347,共5页 Chinese Journal of Otology
关键词 腺相关病毒 血清型 载体 耳蜗 基因转染/基因治疗
分类号 R373.33 [医药卫生—病原生物学] R456 [医药卫生—治疗学]
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参考文献24

  • 1[1]Bowers WJ,Chen X,Guo H,et al.Neurotrophin-3 transduction attenuates cis platin spiral ganglion neuron ototoxicity in the cochlea.Mol Ther,2002,6(1):12-18.
  • 2[2]Kawamoto K,Ishimoto S,Minoda R,et al.Mathl gene transfer generates new cochlear hair cells in mature guinea pigs in vivo.J Neurosci,2003,23(11):4395-4400.
  • 3[3]Cooper LB,Chan DK,Roediger FC,et al.AAV-mediated delivery of the caspase inhibitor XIAP protects against cisplatin ototoxicity.Otol Neurotol,2006,27(4):484-490.
  • 4[4]Luebke AE,Foster PK,Muller CD,et al.Cochlear function and transgene expression in the guinea pig cochlea,using adenovirus-and adeno-associated virus-directed gene transfer.Hum Gene Ther,2001,12(7):773-781.
  • 5[5]Ishimoto S,Kawamoto K,Kanzaki S,et al.Gene transfer into supporting cells of the organ of Corti.Hear Res,2002,173 (1-2):187-197.
  • 6[6]Okada T,Mizukami H,Urabe M,et al.Development and characterization of an antisense-mediated prepackaging cell line for adeno-associated virus vector production.Biochem Biophys Res Commun,2001,288(1):62-68.
  • 7[7]Rabinowitz JE,Samulski RJ.Building a better vector:the manipulation of AAV virions.Virology,2000,278(2):301-308.
  • 8[8]Liu Y,Okada T,Sheykholeslami K,et al.Specific and efficient transduction of cochlear inner hair cells with recombinant adenoassociated virus type 3 vector.Mol Ther,2005,12(4):725-733.
  • 9[9]Zolotukhin S,Potter M,Zolotukhin I,et al.Production and purification of serotype 1,2,and 5 recombinant adeno-associated viral vectors.Methods,2002,28(2):158-167.
  • 10[10]Matsushita T,Elliger S,Elliger C,et al.Adeno-associated virus vectors can be efficiently produced without helper virus.Gene Ther,1998,5(7):938-945.

同被引文献16

  • 1[1]Shi X,Nuttall AL.Upregulated iNOS and oxidative damage to the cochlear stria vascularis due to noise stress.Brain Res,2003,967(1-2):1-10.
  • 2[2]Labbé D,Teranishi MA,Hess A,et al.Activation of caspase-3 is associated with oxidative stress in the hydropic guinea pig cochlea.Hear Res,2005,202(1-2):21-27.
  • 3[3]Sill AM,Stick MJ,Prenger VL,et al.Genetic epidemiologic study of hearing loss in an adult population.Am J Med Genet,1994,54(2):149-153.
  • 4[4]Spicer SS,Schulte BA.Pathologic changes of preshycuais begin in secondary processes and spread to primary processes of strial marginal cells.Hear Res,2005,205(1-2):225-240.
  • 5[5]Daly TM.Overview of edeno-associatod viral vectors.Methods Mol Biol,2004,246:157-165.
  • 6[6]Flotte TR.Gene therapy progress and prospects:recombinant adeno-associated virus(rAAV) vectors.Gene Ther,2004,11(10):805-810.
  • 7[7]Lalwani AK,Walsh BJ,Reiily PG,et al.Development of in vivo gene therapy for bearing disorders:introduction of adeno-associated virus into the cochlea of the guinea pig.Gene Ther,1996,3(7):588-592.
  • 8[8]Lalwani AK,Walsh B J,Carvalho G J,et al.Expression of edenoassociated virus integrated transgene within the mammalian vestibular organs.Am J Otol 1998,19(3):390-395.
  • 9[11]Xiao W,Chirmule N,Berta SC,et al.Gene therapy vectors based on adeno-associated virus type 1.J Virol,1999,73(5):3994-4003.
  • 10[12]Handa A,Muramatsu S,Qiu J,et al.Adeno-associated virus(AAV)-3-based vectors transduce haematopoietic cells not susceptible to transduction with AAV-2-based vectors.J Gen Viral,2000,81(Pt 8):2077-2084.

引证文献1

中华耳科学杂志
2006年 第4期

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