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人arresten因子对内皮细胞与LOVO细胞黏附的抑制 被引量：1

Anti-adhesion of HUVEC to LOVO cell induced by human arresten
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摘要 目的:观察人arresten因子对人脐静脉内皮细胞HUVEC与LOVO细胞黏附的影响,探讨与之相关的黏附分子表达的变化。方法:半定量RT-PCR检测arresten对人脐静脉内皮细胞表达血管细胞黏附分子-1(VCAM-1)mrRNA的影响,Western-hlot检测VCAM-1蛋白表达的变化。孟加拉玫瑰红活细胞染色法观察arresten因子对内皮细胞与LOVO细胞黏附的影响。结果:RT-PCR及Western-blot结果表明VCAM-1在HUVEC细胞中高表达,但经过arresten因子处理的HUVEC细胞,其VCAM-1在mRNA水平及蛋白水平的表达均显著下降(P< 0．05)。孟加拉玫瑰红活细胞染色法显示arresten能抑制HUVEC细胞与LOVO细胞的黏附。结论:arresten因子通过降低内皮细胞VCAM-1的表达抑制内皮细胞与肿瘤细胞的黏附,这可能是其抑制肿瘤转移的一种途径。 OBJECTIVE ： To investigate anti-adhesion of HUVEC to LOVO cell induced by human arresten and measure the expression of VCAM-1 in HUVEC. METHODS：The expression of VCAM-1 in HUVEC lines was measured by semi- quantitative RT-PCR and Western blot, respectively. The adherence of HUVEC cell line to LOVO cell line was detected by using the rose bengal strain. RESULTS： The expression of VCAM-1 both at mRNA level and protein level decreased significantly in HUVEC cell line induced by human arresten （800 ng/mL） （P〈0.05）. Human arresten （800ng/mL） inhibited the adherence of HUVEC cell line to LOVO cell line （P〈0. 05）. CONCLUSIONS： Human arresten inhibits the adherence of HUVEC cell line to LOVO cell line by inhibiting the expression of VCAM-1 in HUVEC cell line, and it may be used to prevent metastasis.
作者 龙淼云 郑启昌 宋自芳 胡青钢 陈立波
机构地区 华中科技大学同济医学院附属协和医院肝胆外科
出处 《中华肿瘤防治杂志》 CAS 2006年第24期1846-1848,共3页 Chinese Journal of Cancer Prevention and Treatment
基金 国家自然科学基金(30271242 30371396)
关键词 结肠肿瘤/病理学 脐静脉/细胞学 内皮 血管/细胞学 血管生成抑制剂/生物合成 细胞粘附分子/血液 colonic neoplasms/pathology umbilical veins/cytology endothelium, vascular/cytology angiogenesis inhibitors/biosynthesis cell adhesion molecules/blood
分类号 R730.2 [医药卫生—肿瘤]
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参考文献6

  • 1Colorado P C,Torre A,Kamphaus G,et al. Anti-angiogenic cues from vascular basement membrane collagen[J]. Cancer Res,2000, 60(9) :2520-2526.
  • 2郑启昌,宋自芳,郑幼伟,喻方敏,李毅清,舒晓刚,宋国英,李振勇.血管生成抑制因子arresten基因的克隆表达[J].中国生物工程杂志,2002,22(4):89-92. 被引量：4
  • 3熊俊,宋自芳,舒晓钢,卢昕,屈新才,郑启昌.瘤内注射pSTbAT-脂质体复合物对裸鼠移植瘤抑制效应的实验研究[J].肿瘤防治杂志,2005,12(1):1-4. 被引量：3
  • 4Blanco M J, Moreno-Bueno G,Sarrio D, et al. Correlation of Snail expression with histological grade and lymph node status in breast carcinomas[J]. Oncogene, 2002,21(5) : 3241 - 3246.
  • 5Lee E, Chae Y, Kim I,et al. Prognostic relevance of immunohistochemically detected lymph node micrometastasis in patients with gastric eareinoma[J]. Cancer, 2002,94(5):2867-2873.
  • 6Calixto J B, Campos M M, Otuki M F, et al. Anti-inflammatory compounds of plant origin. Part Ⅱ. modulation of pro-inflammatory cytokines, chemokines and adhesion molecules[J]. Planta Med,2004,70(2) :93-103.

二级参考文献14

  • 1Colorado P C,Torre A,Kamphaus G,et al. Anti-angiogenic cues from vascular basement membrane collagen [J]. Cancer Res,2000, 60(9): 2520-2526.
  • 2Dabrosin C, Palmer K, Gauldie J. Oestradiol enhances tumour regression induced by B7-1/IL-2 adenoviral gene transfer in a murine model of breast cancer[J]. Br J Cancer, 2003, 89(2) : 385-390.
  • 3MaHI, LinSZ, ChiangYH, etal. Intratumoral gene therapy of malignant brain tumor in a rat model with angiostatin delivered by adenoassociated viral (AAV) vector[J]. Gene Ther, 2002, 9(1): 2-11.
  • 4Nishikawa M, Huang L. Nonviral vectors in the new millennium: delivery barriers in gene transfer[J]. Hum Gene Ther, 2001, 12(8) : 861-870.
  • 5Lee M J, Cho S S, You J R,et al. Intraperitoneal gene delivery mediated by a novel cationic liposome in a peritoneal disseminated ovarian cancer model[J]. Gene Ther, 2002, 9(13): 859-866.
  • 6Noga Yerushalmi, Ulrich Brinkmann, Elisabeth Brinkmann, et al. Attenuating the growth of tumors by intratumoral administration of DNA encoding Pseudomonas exotoxin via cationic liposomes[J]. Cancer Gene Therapy, 2000,7(1) : 91-96.
  • 7Folkman J.Clinical application of research on angiogenesis.N Engl J Med,1995, 333, 1757～1763
  • 8Holmgren L O'Reilly MS,Folkman J.Dormancy of micrometastasis: balanced proliferation and apoptosis in the presence of angiogenesis suppression.Nat Med,1995, 1: 149～153
  • 9Folkman J.What is the evidence that tumors are angiogenesis-dependent? J Natl Cancer Inst,1990, 82: 4～6
  • 10Colorado PC,Torre A,Kamphaus G,et al.Anti-angiogenic cues from vascular basement membrane collagen.Cancer Res,2000, 60: 2520～2526

共引文献5

同被引文献7

  • 1龙淼云,郑启昌,宋自芳,胡青钢,张勇.arresten在CHO细胞的表达及其生物学效应[J].中华肿瘤杂志,2007,29(4):249-252. 被引量：2
  • 2Folkman J. Angiogenesis: Therapeutic implication[J].N Engl J Med,1971, 285(5): 1182-1186.
  • 3De la Torre N G, Turner H E, Wass J A. Angiogenesis in prolactinomas: regulation and relationship with tumour behaviour[J]. Pituitary, 2005, 8(1):17- 23.
  • 4Roguin A, Levy A P. Angiogenesis --an update[J]. Peditar Endocrinol, 2005, 2(3):391- 398.
  • 5Colorado P C, Torte A, Kamphaus G, et al. anti-angiogenic cues from vascular basement membrane collagen[J].Cancer Res, 2000, 60(8):2520-2526.
  • 6Yamaguchi H, Ishii E, Tashiro K, et al. Role of umbilical vein endothelial cells in hematopoiesis[J]. Leuk Lymphoma, 2003, 31(1):61- 69.
  • 7Mazzanti C M, Tandle A, Lorang D, et al. Early genetic mechanisms underlying the inhibitory effects of endostatin and fumagillin on human endothelial cells[J]. Genome Res, 2004, 14(8):1585-1593.

引证文献1

中华肿瘤防治杂志
2006年 第24期

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