三七总皂甙诱导脐血单个核细胞分化为内皮细胞

Effect of Total Panax Notoginseng Saponins on Inducing Differen-tiation of Mononuclear Cells in Human Cord Blood into Endothelial Cells

为了研究三七总皂甙(total panax notoginseng saponins,tPNS)对脐血单个核细胞诱导出内皮细胞的影响,采用含三七总皂甙的DMEM对脐血单个核细胞进行诱导,用光学显微镜和荧光显微镜观察细胞形态,流式细胞术检测细胞表面标记UEA-1、功能标志vWF及CD31的变化。结果显示:250mg/L tPNS组诱导所得的贴壁细胞数及结合UEA-1的阳性率均多于其它浓度的三七总皂甙组(P<0.05)。50ng/ml VEGF+250mg/L tPNS组表达CD31和结合UEA-1阳性的贴壁细胞数与50ng/ml VEGF组相比较,无明显的差异(P>0.05)。结论:中药三七总皂甙可以诱导脐血部分单个核细胞分化为内皮细胞;合用三七总皂甙和VEGF无协同诱导作用。

To investigate the effect of total panax notoginseng saponins （tPNS） to induce the differentiation of mononuclear cells （MNC） in cord blood into endothelial cells, the DMEM culture media containing tPNS were used to induce the MNC of cord blood. Then, the morphology of the adherent cells was observed by the light microscopy and the fluorescence microscopy, the changes of cell surface markers （UEA-1）, function marker （vWF） and CD31 were detected by flow cytometry. The results showed that the number of adherent cells produced by 250 mg/L tPNS and the positive rate of cells expressing CD31 and UEA-1 were higher than those in the groups of other concentrations（ P 〈 0. 05）. There was no significant difference in the number of adherent cells expressing CD31 and UEA-1 between 50 ng/ ml VEGF ＋ 250 mg/L tPNS and 50 ng/ml VEGF. It is concluded that the traditional Chinese drug tPNS can induce partial MNC in the cord blood to differentiate into endothelial cells. No synergistic effect has been found between tPNS and VEGF.