BA-ELISA法用于血清中白喉抗毒素检测的实验探讨

The Experimental Approach on the Detection of Diphtheria Antitoxin in Serum by means of BA - ELISA Method

５６例精白类免疫前后配对血清，用ＢＡ－ＥＬＩＳＡ、常规ＥＬＩＳＡ和ＰＨＡ等三种方法作平行比较试验，其结果：ＰＨＡ法ＧＭＴ免疫前１．８３；免疫后６２．４３，抗体滴度升高３４．１倍；常规ＥＬＩＳＡ法ＧＭＴ免疫前４．８６；免疫后３４０．８，抗体滴度升高７０．０５倍；ＢＡ－ＥＬＩＳＡ法ＧＭＴ免疫前９．０５；免疫后１０３７．０５，抗体滴度升高１１４．６倍。以免疫后ＰＨＡ法ＧＭＴ６２．４３作为敏感度１。

The parallel comparative tests were performed on purifided diphtheria toxoid sera of 56 cases before and after immunization by three methods: BA- ELISA, regular ELISA and PHA. The results were as follow: By PHA method, GMT was 1.83 before immunization and 62.43 after it. The titre of antibody increased 34. 1 times. By regular ELISA method, GMT was 4. 86 before immunization and 340. 8 affer it. The antibody titre increased 70.05 times. By BA- ELISA method, GMT was 9. 05 before immunization and 1037. 05 affer it. The antibody titre increased 114. 6 times. Taken GMT 62. 43 after immunization by PHA method as susceptibility 1, then regular ELISA method was 5.46 times more sensitive than PHA method; The susceptibility of BA - ELISA method was 3.042 times higher than that of regular ELISA method. BA - ELISA method was satisfactory when used to detect diphtheria antitoin in serum.