自体骨髓单个核细胞移植促进移植静脉桥血管再内皮化及抑制内膜增生

Auto-bone marrow mononuclear cells transplantation accelerates reendothelialization and inhibits neointima formation in vein grafts

目的：探讨自体骨髓单个核细胞（bone marrow mononuclear cells，BMMNCs）移植对自体静脉移植后静脉桥血管再内皮化及内膜增生的作用。方法：抽取成年兔骨髓分离制备BMMNCs，并用DAPI进行体外标记。将成年新西兰大白兔23只随机分为细胞移植组（n=13）和对照组（n=10）2组。取左颈外静脉4～5cm，上下倒置后移植至颈总动脉，在建模后第3天将100μl DAPI标记的BMMNCs液（6×10^8个细胞）经耳缘静脉注入细胞移植组动物体内，对照组注入等量的PBS液。细胞移植4周后处死动物，计算机图像分析系统测量并计算静脉移植段血管内皮的完整性及内膜厚度。结果：移植静脉血管内皮有DAPI标记的细胞；细胞移植后，细胞移植组移植血管内皮细胞覆盖程度明显高于对照组（P〈0．05），内膜厚度明显低于对照组（P〈0．05）。结论：自体BMMNCs的移植可以促进移植静脉桥血管的再内皮化及抑制内膜的过度增生。

Objective： To assess the effect of auto bone marrow mononuclear cells （BMMNCs） transplantation on reendothelialization and neointima formation in vein grafts. Methods： BMMNCs were extracted from the bone marrows of adult rabbit under sterile environment and were labelled with DAPI before transplanted into vein grafts. Twenty adult rabbits were randomly divided into 2 groups： BMMNCs transplantation group（group Ⅰ ,n= 10） and PBS transplantation group （group Ⅱ , n= 10）. The left external jugular vein of 20 rabbits were harvested and transplanted between ipsolateral common carotid artery （AVG）. Three days later, animals in group Ⅰ were transplanted with BMMNCs（6×10^8 cells）/100μl via periotic veins and those in group Ⅱ were injected with 100 μl PBS. Animals were killed 4 weeks later and graft veins were harvested to observe the reendothelialization and the thickness of vein grafts. Results： We found that the transplanted cells survived and were incorporated into the endothelium of vein grafts in group Ⅰ. The endothelium integrity of the vein grafts in group Ⅰ was significantly better than that of group Ⅱ. The intima thickness of vein grafts in group Ⅰ was significantly thicker than that of groupU. Condusion.. BMMCs transplantation therapy may improve reendothelialization of the vein graft and inhibits intimal hyperplasi the vein graft.