应用氪离子激光建立大鼠脉络膜新生血管模型

An Experimental Study on Model Foundation of Choroidal Neovascularization Induced by Krypton Laser in Rats

目的:探讨应用氪离子激光建立棕色挪威(brown Norway,BN)大鼠脉络膜新生血管(Choroidal neovascularization,CNV)模型的有效性和安全性,并观察CNV形成后的变化规律。方法:选取雄性BN大鼠25只,从中随机选取1只大鼠(2只眼)为空白对照,不进行激光光凝;其余24只大鼠48只眼作为实验组,行视网膜光凝。氪离子激光光凝后3、7、14、21、28及56d分别随机抽取4只实验组大鼠(8只眼),行视网膜荧光血管造影(Fundusfluoresceinangiography,FFA)、组织病理及透射电镜检查。结果:经FFA和病理组织学检查证实,光凝后第7天光凝斑部位开始出现荧光素渗漏(59%),21d荧光素渗漏达高峰(84%);21d后荧光素渗漏稳定。光凝后7￣21dCNV最大中央厚度显著增加,21d后无明显改变。结论:应用氪离子激光建立BN大鼠脉络膜新生血管模型安全有效,成模时间短,持续时间长,成模率高。

Objective： To study the efficacy and safety of the model foundation of choroidal neovascularization （CNV） induced by krypton laser in Brown Norway （BN） rats, and observe the trend of the formation and change of CNV after laser photoeoagulation. Methods： Twenty-five male BN rats were used for this study. Two eyes of one rat without any laser photoeoagnlation was randomly selected as the control group and the other forty-eight eyes of twenty-four rats with laser photocoagulation were as the experimental group. Eight eyes of four rats were randomly selected to receive the examination of ftmdus fluorescein angiography （FFA）, histopathology and transmission electron microscopy 3, 7, 14, 21, 28 and 56 days after laser photoeoagulation. Results： After laser photoeoagulation, the leakage appeared in burns on the seventh day （59%）, reached the peak on the twenty-first day （84%）, and kept stable after twenty-one days proved by FFA and histopathology. The central thickness of CNV significantly increased from the seventh day to the twenty-first day and kept stable after twenty-one days. Conclusion： The experimental model of choroidal neovascularization can be induced by krypton laser in BN rats with a stable, safe, long lasting method and using a short time to receive a high successful rate.