兔肝VX2肿瘤经肝动脉碘油化疗栓塞后早期肿瘤细胞凋亡

Early tumor cell apoptosis of rabbit liver VX2 tumor after transhepatic artery lipoidol- chemoembolization treatment

目的研究兔VX2肝肿瘤在经肝动脉碘油抗癌药化疗栓塞（Lp-THAE）后早期的肿瘤细胞凋亡。方法27只新西兰大白兔瘤组织块种植肝VX2肿瘤，均分为经LP-THAE组、灌注抗癌药（THAI）组、灌注生理盐水组，选取处理后24、72、120h观察点，肿瘤外周带和中心带取材、HE和MG-P染色光镜法计算凋亡指数（AI）。结果Lp-THAE引起的肿瘤凋亡为THAI法的（3．42±1．29）倍，THAE肿瘤中心部位的凋亡指数为外周带的（4．52±0．95）倍。结论诱导早期肝癌细胞凋亡是LP-THAE主要的治疗机制。

Objective To study the early tumor cell apoptosis with rabbit liver VX2 tumor implantation model after transhepatie artery lipoidol ehemoem- bolisation（Lp-THAE） treatment. Methods Tween-seven New Zealand white rabbits implanted with liver VX2 tumor were divided into three groups randomly： Lp-THAE, THAI, and control groups. The apoptosis index（M） was evaluated with HE and MGP stainings. Results The Al for Lp-THAE was （3.42 ± 1.29） times as THAI, and the Al of tumor centre areas was （4.52 ±0. 95） times as that of tumor peripheral areas. Conclusion To induce early tumor cell apoptosis is a main mechanism of Lp- THAE treatment.