流感病毒治疗小鼠腹水瘤的实验研究

STUDY ON TREARMENT OF ABDOMINAL HYGROMA WITH INFLUENZAL VIRUS IN MICE

甲型流感病毒７５－３９株鼠肺适应型经鼻腔感染小鼠，可使小鼠致病并发生死亡，ＬＤ５０为３．６７。而经皮下和腹腔感染无致病性，存活率１００％。基于经腹腔注射无致病性的实验结果，进而用此病毒治疗Ｓ＿（３７）腹水瘤小鼠，存活率达９３．３％。体外流感病毒感染Ｓ＿（３７）肿瘤细胞，经不同时间观测，至第３天时Ｓ＿（３７）细胞经台盼蓝染色，发现细胞１００％死亡，而对照组Ｓ＿（３７）细胞死亡率为１０％左右（Ｐ＜０．０１）。进一步研究病毒免疫治疗Ｓ＿（３７）腹水瘤小鼠的机理，发现经病毒感染后小鼠ＮＫ细胞杀伤活性升高达５８％，正常小鼠ＮＫ活性为２２％，两者有显著性差异（Ｐ＜０．０１）。同时，检测注射病毒后小鼠腹腔巨噬细胞的吞噬活性也随之升高。

The mouse lung adaptive type influenza virus A(75-39 strain) was fatal to the mouse infected through the nasal cavity;but it was non-pathogenic when itinfected the mouse subcutaneously or abdominally.The survival rate of the mice bearingabdominal hygroma(S_(37))treated with virus immunotherapy was 93.3%.The death rateot hygroma(S_(37))cells infected with the inflienza virus was 100%by the third day afterthe infection;and that of the control group was about 10%. The research on themechanism of virus immuno therapy used for treatment of mice bearing abdominalhygroma(S_(37))showed that NK cell activity of mice infected by the influenza virus wasas high as 58%at the peak hour,but that of the normal mice was 22%(R<0.01).The phagocytosis of abdOminal macrophages of the mice intected with the virus wasincreased obviously.