聚合酶链反应检测HBV─DNA及临床意义的再认识

DETECTION OF HBV-DNA BY PCR AND RECOGNITION OF ITS CLINICAL SIGNIFICANCE

采用聚合酶链反应检测１１４例肝病患者血清ＨＢＶ－ＤＮＡ。结果：１１４例患者血清ＨＢＶ－ＤＮＡ阳性率３５．９６％，其中ＡＨ、ＣＰＨ、ＣＡＮ、ＬＣ组ＨＢＶ－ＤＮＡ阳性率分别为４１．６７％、２１．４３％、５３．６６％和２６．３１％；ＨＢｅＡｇ（＋）组３９．０２％，抗ＨＢｃ（＋）组２２．７３％，ＨＢｓＡｇ（＋）、抗ＨＢｃ（＋）组４３．３７％，抗ＨＢｓ（＋）组４１．１８％；ＨＢＶＭ（－）组１１．１１％。结果提示：①ＣＡＨ组阳性率较高（５３．６６％）；②抗ＨＢｅ（＋）患者血清中有高滴度ＨＢＶ－ＤＮＡ，提示仍有传染性；③抗ＨＢｅ（＋）、抗ＨＢｓ（＋）、ＨＢＶＭ（－）的患者ＨＢＶ－ＤＮＡ阳性可能为ＨＢＶ的变异株感染。

The serum HBV-DNA were determined by polymerase chain reaction(PCR)in 114 cases of hepatic diseases , the positivity rate of serum HBV-DNA being35.96%.The positivity rates in the AH,CPH,CAH and LC group were 41.7%,21.42%,53.66%and 26.84%respsctively.the posivity rate was 39.02%in the HBeAg(+)group,22.73%in the anti-HBe(+)group,47.37%in the HBsAg(+)and anti-HBc(+)group,41.18%in the anti+HBs(+)group, and 11. 11%in the HBVM(-)group.Theresults reveal that the posivity rate of serum HBV-DNA in the CAH group is the highest(53.66%)in the clinical type groups;there is high titer HBV-DNA in serum ot anti-HBe(+)group,suggesting that tne infectivity exists yet;patients in the anti-HBe(+),anti-HBs(+)and HBVM(-)group whose serum HBV-DNA test is positive may be infected byvariant HBV.