N-乙酰半胱氨酸对大鼠脑出血后核因子-кB表达的影响

The effect of N-acetylcysteine on brain water content and expression of NF-кB in cerebral tissue after experimental intracerebral hemorrhage in rats

目的探讨N-乙酰半胱氨(N-acetylcysteine,NAC)在大鼠脑出血(ICH)继发脑损伤中的作用机制。方法采用立体定向技术注入自体不凝血制作实验性ICH模型,造模后即刻和6h后用NAC干预,行免疫组化染色观察血肿周围脑组织核因子кB(NF-кB)和细胞间黏附分子-1(ICAM-1)的表达变化。结果ICH后6h血肿周围脑组织NF-кB开始表达,48h达高峰,ICAM-l12h开始表达,72h达高峰。各组与假手术组之间有显著差异(均P<0.05);脑出血后NF-кB与ICAM-l呈正相关(r=0.868,P<0.05),NF-кB表达从开始到高峰均早于ICAM-l。NAC可明显降低ICH24h、72h亚组NF-кB、ICAM-l的表达(P<0.05)。结论ICH时,NAC可通过抑制NF-кB的活性,下调ICAM-l的表达,从而发挥脑保护作用。

Objective To investigate the effect and mechanism of N--acetylcysteine on brain water content perihemotoma after experimental intracerebral hemorrhage in rats. Methods Experimental intracerebral hemorrhage model was maded by injection autologous blood using stereotactic method. NAC was administered immediately after ICH. The expression of NF-- κB in cerebral tissues were detected by using immunohistochemistry techniques. At the due time, the rats were killed and brain tissue were removed for measurement of brain water content. Results NF--κ B expression and brain water content increased obviously after ICH. Both can be reduced by NAC treatment. There was obvious difference among each intracerebral hemorrhage group and controls（all P 〈 0. 05） , Conclusions NAC can reduce brain water content after ICH by inhibiting NF-- κ B and subsequent inflammation.