溶血磷脂酸和氧化低密度脂蛋白的相关性分析及与缺血性脑血管病早期的关系

Correlation analysis between plasma lysophosphatidic acid and oxidized low density lipoproteion and their relationship with early lschemic cerebrovascular disease

目的探讨溶血磷脂酸(LPA)和氧化低密度脂蛋白(ox-LDL)的相关性,进一步研究二者在缺血性脑血管病中的变化,揭示其在缺血性脑血管病早期的预警作用。方法随机选择62例患者,根据病情将其分为急性脑梗死早期(CI)组30例,短暂脑缺血发作组(TIA)32例,并选30例健康人作为对照组,分别用ELISA法测定氧化低密度脂蛋白(ox-LDL),用有机溶剂抽提法测定溶血磷脂酸(LPA)。检测结果进行统计学分析。结果急性脑梗死早期组和短暂脑缺血发作组血浆中LPA和ox-LDL水平显著高于对照组(P<0.05),短暂脑缺血发作组与急性脑梗死早期组相比,短暂脑缺血发作组血浆中LPA水平较高(P<0.05)。急性脑梗死早期组和短暂脑缺血发作组血浆中LPA和ox-LDL之间比较呈显著正相关(P<0.05)。结论在缺血性脑血管病早期血浆中LPA和ox-LDL活性增高,且二者之间呈显著正相关,短暂脑缺血发作组血浆中LPA水平较急性脑梗死早期组增高,LPA作为体内凝血和血栓形成过程早期释放的分子标记物,在预测缺血性脑血管病的发生发展,及指导合理应用抗血小板及抗凝药物方面可能有一定应用前景,ox-LDL与缺血性脑血管病的发病也密切相关。

Objective To clarify the role of predicting in early ischemic cerebrovascular disease through investigating the relationship between the level of plasma lysophosphatidic acid （LPA） and oxidized low density lipoproteion （ox--LDL） and observing the changes of LPA and ox--LDL in patients with ischemic cerebrovascular disease. Methods 60 patients with ischemic cerebrovascular disease were divided into two groups： 28 with acute cerebral infarction and 32 with vertebrobasilar transient ischemic attack, as well as 32 healthy people （control group）. LPA and ox--LDL were determined by organic solvent extraction and ELISA respectively. Results LPA and ox--LDL were significantly higher than that in normal controls . The LPA level in transient ischemic attack group was higher compared with that of early acute cerebral infarction group. There was a positive relationship between the degree of LPA and ox-- LDL in early ischemic cerebrovascular group and transient ischemic attack group. Conclusions Cor- relation analysis showed LPA was positively correlated with ox--LDL level in patients with early ischemic cerebrovascular disease. Plasma LPA level in transient ischemic attack group was obviously higher than that in patients with early acute cerebral infarction group. As a molecular marker released by activated platelets in vivo, plasma LPA may be valuable in predicting occurrence and development of ischemic cerebrovascular disease and directing application of antiplatelet and anticoagulant therapy, ox--LDL also has an association with ischemic cerebrovascular disease.