鸡胚胎干细胞的分离、培养与鉴定的初步研究

Pilot study of isolation,culture and identification of stem cells in chicken embryo

采用饲养层培养法,以高糖DM EM为基础培养基,同时添加胎牛血清以及5 ng/m l SCF,l0 ng/m l bFGF和1 000 IU/m l mL IF等细胞因子与鸡胚浸出液等,对鸡的X期胚盘细胞进行离体培养,并利用形态学鉴定、AKP染色、体外分化实验等方法对所获得的细胞克隆进行鉴定。实验结果表明,本实验建立的培养体系培养出了具有多分化潜能的类胚胎干细胞。

The feeder layer was used to culture the embryonic stem cells. The X-stage blastoderm cells were cultured in vitro with DMEM （high glucose） as base culture medium supplemented with fetal calf serum, 5 ng/ml SCF, 10 ng/ml bFG, 1 000 IU/ml mLIF. The cell clone was identified by morphologic method, AKP dying and differentiation experimentation in vitro . The results indicated that the pluripotency stem cells can be obtained in a suitable culture system built in this study.