肺癌患者血管内皮生长因子和T细胞亚群表达的变化

Changes of VEGF and T Cell Subsets Expression in Patients with Lung Cancer

测定和比较83例肺癌患者与60名正常人血清血管内皮生长因子(VEGF)和T细胞亚群(CD3+、CD4+、CD8+、CD8+CD28+)和NK细胞的表达水平,探讨其临床意义,VEGF采用ELISA法,T细胞亚群的表达采用流式细胞仪法。结果表明:肺癌患者VEGF水平明显高于对照组(P<0.01)。CD4+细胞、CD8+CD28+细胞和NK细胞明显低于对照组(P<0.01)。CD8+细胞则明显高于对照组(P<0.01)。提示肺癌患者血清VEGF增高和细胞免疫功能紊乱,有一定的临床意义。

To investigate the expression of vascular endothelial growth factor （VEGF） and T cell subsets in blood of patients with lung cancer and to evaluate the clinical significance, serum level of VEGF in 83 patients with lung cancer and 60 normal controls was measured by EIASA, lymphocyte subsets（CD3^＋ ,CD4^＋ ,CD8^＋ , CD8^＋ CD28^＋ ）and NK cell by flowcytometry. Serum VEGF level in patients with lung cancer was significantly higher than that in control group （P 〈0.01）. Percentage of CD4^＋ cell,CD8^＋ CD28^＋ cell and NK cell in patients with lung cancer decreased significantly compared with control group （P〈0.01）, Percentage of CD8^＋ cell in patients with lung cancer increased significantly compared with control group （36.8±7.6% VS 31.8±5.1%,P〈0.01）. It suggested that the high level of serum VEGF and dysfunction of cellular immunity in patients with lung cancer may have clinical significance.