摘要
探讨肿瘤化疗药物表阿霉素(E-ADM)对K562细胞的杀伤作用及其可能的机制。采用流式细胞术对经不同浓度、不同时间E-ADM作用的K562细胞株的自发荧光(SF)、细胞死亡率(PI)及多药耐药基因蛋白(P170)的细胞表达百分率及其表达的平均荧光强度(MFI)进行检测和分析。在E-ADM浓度为0.01μg/mL时,SF+、PI+及P170+细胞即开始上升,后迅速升高,杀伤效果呈现出典型的“S”形曲线分布,24h组的各项指标结果均显著高于72h组。K562细胞在不同浓度E-ADM作用后,SF+、PI+及P170+细胞百分率及其表达的MFI均随E-ADM浓度的增加而增高;3个指标的细胞阳性百分率与E-ADM浓度之间无显著的相关性(P均>0.05),而其MFI与E-ADM浓度之间却有显著的正相关(P均<0.01)。E-ADM对K562细胞有明显的杀伤作用,其杀伤效果与E-ADM浓度呈正相关,E-ADM杀伤K562细胞存在一个杀伤效应的剂量敏感区、一个最佳杀伤浓度和最适作用时间,该结果可为E-ADM的临床应用提供有价值的参考依据。
To explore killing effect of E-ADM on K562 cell strain and its possible mechanism, the percentage and mean fluorescence intensity （MFI） of cell spontaneous fluorescence（SF^＋ ）, cell mortality rate （PI^＋ ）, and P170 expression of K562 cell strain were analyzed by flow cytometry after co-incubation with E-ADM by different concentration and different time span. The positive rate of SF^＋ , PI^＋ and P170 began to increase at 0.01μg/mL of E-ADM, and increased immediately accompanied effecting curve as a ＂S＂ type, and effect of 24h were superior to those of 72h. The positive rate of SF^＋ , PI^＋ and P170 expression increased with concentration of E- ADM, though without significant positive correlation（P〉0.05）. Significant positive correlation existed between MFI of SF^＋ , PI^＋ and P170 expression and concentration of E-ADM （P〈0.01）, as well as among three markers（P〈0.01）. E-ADM has obvious killing effect on K562 with a dosage sensitive region ,optimistic killing concentration and the most suitable incubating time, which is positively related to E-ADM concentration. Results can provide valuable reference evidence for clinical application of E-ADM.
出处
《标记免疫分析与临床》
CAS
2006年第4期227-230,共4页
Labeled Immunoassays and Clinical Medicine
基金
江苏省科技厅社会发展项目资助(BS99027)
