银杏内酯B注射液对大鼠脑缺血再灌注损伤的保护作用

Effect of Ginkgolide B Injection on rat cerebral ischemia reperfusion lesion

目的探讨银杏内酯B对大脑局灶性缺血再灌注模型大鼠的保护作用及可能的机制。方法采用栓线法大鼠缺血3 h再灌注21 h模型,观察银杏内酯B对模型大鼠神经症状、梗死范围及脑组织匀浆中超氧化物歧化酶(SOD)、谷胱甘肽过氧化酶(GSH-PX)、钠-钾ATP酶(Na+-K+-ATPase)、钙-镁ATP酶(Ca2+-My2+-ATPase)活性及丙二醛(MDA)、过氧化氢酶(CAT)、谷胱甘肽(GSH)、一氧化氮(NO)含量的影响。结果银杏内酯B注射液8、4 mg/kg组可明显改善大鼠神经症状,减轻梗塞程度,抑制率分别为29.6%、23.4%(与模型组比较P<0.01,P<0.05)。银杏内酯B注射液8、4 mg/kg可明显升高缺血再灌侧大脑的SOD、GSH-PX活性,提高GSH、CAT含量,降低MDA、NO含量,明显升高缺血再灌侧大脑的Na+-K+-ATPase、Ca2+-My2+-ATPase的活性(P<0.05,P<0.01)。结论银杏内酯B对局灶性脑缺血再灌注损伤大鼠具有保护作用,本作用与银杏内酯B减少大鼠脑缺血再灌注后脑组织NO含量、抗自由基损伤以及改善能量代谢有关。

Objective To investigate the protective effect and the mechanism of Ginkgolide B Injection on rat model of brain local ischemia reperfusion lesion. Method The rat model of ischemia （3 hours） and reperfusion （21 hours） induced by thread approach were established for observing the effect of Ginkgolide B Injection on the neurological symptoms, infarction size, the activities of SOD, GSH-PX, Na ^＋ -K ^＋ -AT- Pase and Ca^2＋-My^2＋ -ATPase, and the contents of MDA, CAT, GSH and NO. Result Ginkgolide B Injection in the doses of 8 mg/kg and 4 mg/kg relieved obviously the neurological symptoms and infarction in rats, the inhibitory rates were 29.6% and 23.4% respectively （ P 〈 0. 01, P 〈 0.05 compared with those of the model group）, meanwhile improved the activities of SOD and GSH-PX, increased the contents of GSH and CAT, decreased MDA and NO contents, and promoted obviously the activities of Na^＋ -K ^＋ -ATPase and Ca^2＋ -My^2＋ -ATPase （P 〈0. 05, P 〈0.01 ） in the brain after ischemia and reperfusion lesion. Conclusion Ginkgolide B Injection has the protective effect on rats with cerebral local ischemia and reperfusion lesion, which may be related to that Ginkgolide B Injection can decrease NO content, antagonize lesion of free radicals and improve energy metabolism.