淡红蜡伞子实体水溶性粗多糖提取方法探索

Optimization of Conditions for Extracting Crude Polysaccharide from Hygrophorus russula

以多糖得率为指标,对淡红蜡伞[Hygrophorus russula(Fr.)Qu啨l.]子实体预处理方式和提取次数进行了比较,并采用正交试验,进一步优化淡红蜡伞多糖提取的条件,结果表明淡红蜡伞干燥子实体提取粗多糖较佳的方法是:干燥子实体粉碎过60目筛,以蒸馏水为提取剂,提取2次可得到约4.3%的粗多糖;正交试验表明,各因素对淡红蜡伞多糖得率的影响程度依次为乙醇沉淀浓度、料水比、提取温度和提取时间,最佳提取条件是提取时间4h,温度80℃,料水比1∶10,乙醇沉淀浓度85%,在此条件下多糖得率为6.52%。

The nature of the starting materiat （i, e. powdered versus chipped fruit bodies of Hygrophorus russula） and increasing extraction frequencies were compared using polysaccharide yields as an index of extraction efficiency. Higher polysaccharide yields were obtained when extractions were carried out using powdered H. russula fruit bodies compared with fruit body chips, and crude polysaccharide yields of almost 4.3% were obtained from powdered material after two consecutive extractions using distilled water. Application of orthogonat testing to optimize extraction conditions revealed that polysaccharide yields were influenced mainly by the ethanol concentration used for precipitation, followed by temperature, solid：liquid ratio and treatment time. The optimal conditions were., extraction time 4 h, temperature 80 ℃, solid：liquid ratio 1：10, and ethanol concentration 85%, Under these conditions, a polysaccharide yield of 6, 52% was obtained.