摘要
分子标记辅助选择逐步成为植物育种的重要方法。然而需要更多的实用的分子标记,尤其是与重要农艺性状紧密连锁的标记。目前,许许多多的方法已经应用到棉花结构基因组的标记开发,但是棉花SNP标记鲜见报道。FIF1基因是棉纤维优势表达的一个基因,研究证明它在棉纤维的发育过程中起着很重要的调控作用。根据已发表的亚洲棉FIF1基因,设计特异引物,克隆了陆地棉TM-1和海岛棉海7124中FIF1基因。根据2个四倍体棉花FIF1基因的SNP变异,采用SNAP和CAPs方法将该基因定位到四倍体棉花遗传图谱上。研究表明棉花SNP标记的开发是可行的,也是有效的。
Marker-assisted selection （MAS） has been an important way for practical plant breeding, and more available markers are required, especially those linked to the genes of some important agronomical traits. Many kinds of molecular markers are being used in cotton genetic mapping, but there is little report on SNP markers development of cotton genes. FIF1 is predominantly expressed early in developing cotton fibers and identified to be a key regulator of cotton fiber development. In this paper, FIF1 genes from G. hirsutum cv. TM-1 and G. barbadense cv. Hai7124 were cloned on the basis of its published sequence in G. arboreum L. Based on the SNPs between FIF1 genes from two tetraploid cotton, SNP and CAPs markers were developed, and the gene was mapped on our allotetraploid cotton genetic map. This result indicates that SNP marker development is feasible and effective in Gossypium.
出处
《作物学报》
CAS
CSCD
北大核心
2007年第2期256-261,共6页
Acta Agronomica Sinica
基金
This program was financially supported in part by the National High Technology Research and Development Program of China(863 Program)(2004AA211172),The National Basic Research Program(973 Program)(2002CB111301),the Key Project of Chinese Ministry of Education(10418),and the program for Changjiang scholars and the innovative research teams in universities.
