小麦叶片氮含量与荧光动力学参数的关系

Relationship between Leaf Nitrogen Contents and Fluorescence Parameters in Wheat

通过分析不同氮素水平下不同品种小麦上部叶片氮含量和荧光参数随生育进程的变化模式及小麦叶片氮含量与荧光参数的相关关系,提出小麦叶片氮含量的预测方程。结果表明,小麦不同叶位叶片氮含量和荧光参数Fv/Fm、Fv/Fo均随施氮量的增加呈上升趋势。从孕穗到成熟,Fv/Fm和Fv/Fo表现为先上升后下降的趋势。小麦各叶位叶片氮含量与荧光参数Fm、Fv、Fv/Fm、Fv/Fo、Fs和Fo′都呈极显著正相关,且顶部2张叶片的相关性最好。不同小麦品种顶2叶氮含量分别与Fv/Fo和Fv/Fm呈良好的幂函数关系,平均决定系数分别达0.66和0.61。对于低、中、高蛋白质含量的小麦品种类型,可以利用统一的回归方程来描述顶部2张叶片氮含量随Fv/Fo的变化模式。

It is very important for using non-destructive method to determine nitrogen status and growth characters of crop plants. Remote sensing has been successfully applied in assessing crop biophysical and biochemical variables, such as leaf area index, chlorophyll, nitrogen and so on. Chlorophyll fluorescence is a new non-destructive instrumentation for predicting leaf nitrogen content of crop. In this study, two experiments were carried out at Nanjing to investigate the correlative relationship of leaf nitrogen content to fluorescence parameters in wheat （ Triticum aestivum L. ）. Experiment one was conducted with two ctdtivars （Ningmai 9 and Huaimai 20） and five nitrogen rates of 0, 75, 150, 225, and 300 kg ha^-1 in 2003. Experiment two included two cultivars （Ningmai 9 and Yumai 34） with three nitrogen rates of 0, 90, and 270 kg ha^-1 in 2004. The change patterns of leaf nitrogen contents and fluorescence parameters across growth stages in different wheat cuhivars were determined under different nitrogen levels, the correlative relationships of leaf nitrogen contents to fluorescence parameters were established, and the regression equations for predicting leaf nitrogen contents were formulated. The results showed that the leaf nitrogen contents and fluorescence parameters at different leaf positions of wheat increased with raised nitrogen applications. From booting to maturity, fluorescence parameters Fv/Fm, and Fv/Fo of wheat leaves initially increased and then decreased with growth process of time. leaf nitrogen contents of different leaf positions in wheat had significant positive correlations with fluorescence parameters Fm, Fv, Fv/Fm, Fv/Fo, Fv, and Fo＇, especially the correlation in the 1st and 2nd leaves from top was the best. Nitrogen contents of two top leaves in different wheat cultivars were significantly related to Fv/Fm and Fv/Fo, with average R^2 of 0.66 and 0.61, respectively. For different types of ctdtivar with low, medium and high grain protein to describe the dynamic change pattern of leaf nitrogen contents at contents, an integrated regression equation could be used the two top leaves with fluorescence parameter （ Fv/Fo ）.