一种碱溶性灰树花菌丝体多糖GFM2A的制备和结构表征

Preparation and Structure Characterization of an Alkali-soluble Polysaccharide from Grifola frondosa Mycelium

以灰树花菌丝体为原料,经过碱提取和柱色谱分离纯化,得到一种碱溶性多糖(GFM2A).经高碘酸氧化、Sm ith降解、乙酰解并采用GCMS,IR,2D NMR等方法对该多糖的化学结构进行了表征.结果表明,该多糖由葡萄糖(G lc)、甘露糖(M an)和木糖(Xyl)组成,其摩尔比为9∶2∶1,其主链由6个-α1,3-D-G lc和2个-α1,3-D-M an构成,且-α1,3-D-G lc残基O-4位有两个分支,其中一个分支连接3个-β1,4-D-G lc,另一个分支连接一个-α1,4-D-Xyl.

An alkali-soluble polysaccharide （GFM2A）was extracted by cold sodium hydroxide and further purified by column chromatography from fermented Grifola frondosa mycelium. The structural characterization of GFM2A was confirmed by NaIO4 oxidation, Smith degradation, acetolysis, GCMS, IR and NMR techniques. The results showed that GFMZA contain glucose （Glc）, mannose （Man）and xylose （Xyl）, and their molar ratio was 9： 2： 1. There are six α-1 ,3-D-Glc and two α-1,3-D-Man in the backbone, and there are two branches in the main chain, and both of them linked to α-1 ,3-D-GIc residue, one branch contains three β-1, 4-D-Glc, another branch contains one α-1,4-D-Xyl.