FTIR直接测定法在解决忍冬科一些分类学问题中的应用研究

The Applications of Fourier Transform Infrared Spectroscopy in Resolving Some Taxonomic Doubts of Caprifoliaceae

应用傅里叶变换红外光谱(FTIR)法测定了忍冬科5属12种植物的红外光谱,对其红外光谱及系统关系进行了分析。结果表明:各分类群的红外光谱具有高度特异性和重现性,七子花属与忍冬族和北极花族的关系都比较接近,表明七子花属有可能在一定程度上联系着忍冬族和北极花族,而接骨木属和荚蒾属与科内其他属的距离并不远,支持目前把这两属归入忍冬科的分类方法。说明利用已知的标准红外光谱图库,可以鉴定和区分出这些属或其他属植物的种类及关系。

Fourier transform infrared spectroscopy （FTIR） provides biochemical profiles containing overlapping signals from a majority of the compounds that are present when wholesamples are analyzed. The spectra of the xylem of 112 samples belonging to 12 species and 5 genera in Caprifoliaceae were determined directly with FTIR spectrometry and OMNI-sampler. Based on the indices of wave number -absorbance, the phylogenetic relationships of the 12 species were analyzed by the methods of principal coordinate analysis （PCO） and cluster analysis. The results showed that the infrared spectra of the 12 plant species are fingerprint-like patterns which are highly typical for different taxa. Genus Heptacodium has close relation with the tribe Linnaeeae, and Lonicereae, showing that its systematic position is probably located between the two tribes. There is no obvious difference between Viburnum and Sambucus, so the authors thought it would be better to put the two genera in Caprifoliaceae. Therefore, the infrared spectra are of taxonomic value at the level of species and genera, and this technique could be widely used for identification and classification of other taxa when standard spectra are available.