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phyA基因在毕赤酵母中的表达以及表达产物的检测 被引量:1

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摘要 对获得的植酸酶基因PhyA进行TA克隆,获质粒pTA-phyA,酶切后,使其在连接体系中连接到酵母表达载体pPIC9K-InS片段上,随后将重组质粒转化给大肠杆菌,验证其成功后,再将重组质粒转化给毕赤酵母,诱导其表达,通过测定植酸酶活性,得出其表达量。
作者 曾青兰
出处 《现代农业科技》 2007年第3期105-106,共2页 Modern Agricultural Science and Technology
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  • 1江均平.热稳定的曲霉植酸酶[J].微生物学报,1996,36(6):476-478. 被引量:18
  • 2李杰.-[J].中国动物营养学报,1992,(2):52-52.
  • 3吕忠进.-[J].饲料博览,1992,(2):9-9.
  • 4[1]Sreekrishna K, Brankamp R G, Kropp K E, et al. Strategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeast Pichia pastoris. Gene, 1997, 190: 55~62.
  • 5[2]Clare J J, Scorer C A, Buckholz R G, et al. Expression of EGF and HIV envelope glycoprotein. Methods Mol Biol, 1998, 103: 209.
  • 6[3]Scorer C A, Buckholz R G, Clare J J, et al. The intracellular production and secretion of HIV-1 envelope protein in the methylotrophic yeast Pichia pastoris. Gene, 1993, 136:111~119.
  • 7[4]Zhang Y J, Jin N Y, Jiang W Z, et al. Cloning and expression of the external-glycoprotein gene mutant from HIV-2 in the methylotrophic yeast Pichia pastoris and identification of the glycoprotein. Biotechnol Appl Biochem, 2001, 34:1~4.
  • 8[5]Withers-Martinez C, Carpenter E P, Hackett F, et al. PCR-based gene synthesis as an efficient approach for expression of the A+T-rich malaria genome.Protein Eng, 1999, 12:1113~1120.
  • 9[6]Cregg J M, Cereghino J L, Shi J Y, et al. Recombinant protein expression in Pichia pastoris. Mol Biotechnol, 2000, 16: 23~52.
  • 10[7]Waterham H R, Digan M E, Koutz P J, et al. Isolation of the Pichia pastoris glyceraldehyde-3-phosphate dehydrogenase gene and regulation and use of its promoter. Gene, 1997, 186: 37~44.

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